Detecting respiratory viral RNA using expanded genetic alphabets and self-avoiding DNA
Identifieur interne : 001835 ( Main/Exploration ); précédent : 001834; suivant : 001836Detecting respiratory viral RNA using expanded genetic alphabets and self-avoiding DNA
Auteurs : Lyudmyla G. Glushakova [États-Unis] ; Nidhi Sharma [États-Unis] ; Shuichi Hoshika [États-Unis] ; Andrea C. Bradley [États-Unis] ; Kevin M. Bradley [États-Unis] ; Zunyi Yang [États-Unis] ; Steven A. Benner [États-Unis]Source :
- Analytical Biochemistry [ 0003-2697 ] ; 2015.
Descripteurs français
- KwdFr :
- ADN (métabolisme), ADN simple brin (métabolisme), ARN viral (isolement et purification), ARN viral (métabolisme), Acides nucléiques immobilisés (métabolisme), Biologie synthétique (), Colorants fluorescents (), Coronaviridae (), Coronaviridae (isolement et purification), Coronaviridae (métabolisme), DNA-directed RNA polymerases (métabolisme), Désoxyribonucléosides (métabolisme), Hybridation d'acides nucléiques (), Hétéroduplexes d'acides nucléiques, Infections de l'appareil respiratoire (diagnostic), Infections de l'appareil respiratoire (virologie), Liaison hydrogène, Limite de détection, Microsphères, Orthomyxoviridae (), Orthomyxoviridae (isolement et purification), Orthomyxoviridae (métabolisme), Phycoérythrine (), Protéines virales (métabolisme), Pyridones (métabolisme), RT-PCR, Réaction de polymérisation en chaine multiplex (), Triazines (métabolisme), Typage moléculaire (), Virus respiratoires syncytiaux (), Virus respiratoires syncytiaux (isolement et purification), Virus respiratoires syncytiaux (métabolisme).
- MESH :
- diagnostic : Infections de l'appareil respiratoire.
- isolement et purification : ARN viral, Coronaviridae, Orthomyxoviridae, Virus respiratoires syncytiaux.
- métabolisme : ADN, ADN simple brin, ARN viral, Acides nucléiques immobilisés, Coronaviridae, DNA-directed RNA polymerases, Désoxyribonucléosides, Orthomyxoviridae, Protéines virales, Pyridones, Triazines, Virus respiratoires syncytiaux.
- virologie : Infections de l'appareil respiratoire.
- Biologie synthétique, Colorants fluorescents, Coronaviridae, Hybridation d'acides nucléiques, Hétéroduplexes d'acides nucléiques, Liaison hydrogène, Limite de détection, Microsphères, Orthomyxoviridae, Phycoérythrine, RT-PCR, Réaction de polymérisation en chaine multiplex, Typage moléculaire, Virus respiratoires syncytiaux.
English descriptors
- KwdEn :
- Coronaviridae (classification), Coronaviridae (isolation & purification), Coronaviridae (metabolism), DNA (metabolism), DNA, Single-Stranded (metabolism), DNA-Directed RNA Polymerases (metabolism), Deoxyribonucleosides (metabolism), Fluorescent Dyes (chemistry), Hydrogen Bonding, Immobilized Nucleic Acids (metabolism), Limit of Detection, Microspheres, Molecular Typing (methods), Multiplex Polymerase Chain Reaction (methods), Nucleic Acid Heteroduplexes, Nucleic Acid Hybridization (methods), Orthomyxoviridae (classification), Orthomyxoviridae (isolation & purification), Orthomyxoviridae (metabolism), Phycoerythrin (chemistry), Pyridones (metabolism), RNA, Viral (isolation & purification), RNA, Viral (metabolism), Respiratory Syncytial Viruses (classification), Respiratory Syncytial Viruses (isolation & purification), Respiratory Syncytial Viruses (metabolism), Respiratory Tract Infections (diagnosis), Respiratory Tract Infections (virology), Reverse Transcriptase Polymerase Chain Reaction, Synthetic Biology (methods), Triazines (metabolism), Viral Proteins (metabolism).
- MESH :
- chemical , chemistry : Fluorescent Dyes, Phycoerythrin.
- chemical , isolation & purification : RNA, Viral.
- chemical , metabolism : DNA, DNA, Single-Stranded, DNA-Directed RNA Polymerases, Deoxyribonucleosides, Immobilized Nucleic Acids, Pyridones, RNA, Viral, Triazines, Viral Proteins.
- classification : Coronaviridae, Orthomyxoviridae, Respiratory Syncytial Viruses.
- diagnosis : Respiratory Tract Infections.
- isolation & purification : Coronaviridae, Orthomyxoviridae, Respiratory Syncytial Viruses.
- metabolism : Coronaviridae, Orthomyxoviridae, Respiratory Syncytial Viruses.
- methods : Molecular Typing, Multiplex Polymerase Chain Reaction, Nucleic Acid Hybridization, Synthetic Biology.
- virology : Respiratory Tract Infections.
- Hydrogen Bonding, Limit of Detection, Microspheres, Nucleic Acid Heteroduplexes, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Nucleic acid (NA)-targeted tests detect and quantify viral DNA and RNA (collectively xNA) to support epidemiological surveillance and, in individual patients, to guide therapy. They commonly use polymerase chain reaction (PCR) and reverse transcription PCR. Although these all have rapid turnaround, they are expensive to run. Multiplexing would allow their cost to be spread over multiple targets, but often only with lower sensitivity and accuracy, noise, false positives, and false negatives; these arise by interactions between the multiple nucleic acid primers and probes in a multiplexed kit. Here we offer a multiplexed assay for a panel of respiratory viruses that mitigates these problems by combining several nucleic acid analogs from the emerging field of synthetic biology: (i) self-avoiding molecular recognition systems (SAMRSs), which facilitate multiplexing, and (ii) artificially expanded genetic information systems (AEGISs), which enable low-noise PCR. These are supplemented by “transliteration” technology, which converts standard nucleotides in a target to AEGIS nucleotides in a product, improving hybridization. The combination supports a multiplexed Luminex-based respiratory panel that potentially differentiates influenza viruses A and B, respiratory syncytial virus, severe acute respiratory syndrome coronavirus (SARS), and Middle East respiratory syndrome (MERS) coronavirus, detecting as few as 10 MERS virions in a 20-μl sample.
Url:
DOI: 10.1016/j.ab.2015.08.015
PubMed: 26299645
PubMed Central: 4733849
Affiliations:
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Glushakova</name><affiliation wicri:level="2"><nlm:aff id="aff1">Firebird Biomolecular Sciences, Alachua, FL 32615, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Firebird Biomolecular Sciences, Alachua, FL 32615</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Sharma, Nidhi" sort="Sharma, Nidhi" uniqKey="Sharma N" first="Nidhi" last="Sharma">Nidhi Sharma</name><affiliation wicri:level="2"><nlm:aff id="aff2">Foundation for Applied Molecular Evolution (FfAME), Alachua, FL 32615, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Foundation for Applied Molecular Evolution (FfAME), Alachua, FL 32615</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Hoshika, Shuichi" sort="Hoshika, Shuichi" uniqKey="Hoshika S" first="Shuichi" last="Hoshika">Shuichi Hoshika</name><affiliation wicri:level="2"><nlm:aff id="aff2">Foundation for Applied Molecular Evolution (FfAME), Alachua, FL 32615, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Foundation for Applied Molecular Evolution (FfAME), Alachua, FL 32615</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Bradley, Andrea C" sort="Bradley, Andrea C" uniqKey="Bradley A" first="Andrea C." last="Bradley">Andrea C. Bradley</name><affiliation wicri:level="2"><nlm:aff id="aff2">Foundation for Applied Molecular Evolution (FfAME), Alachua, FL 32615, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Foundation for Applied Molecular Evolution (FfAME), Alachua, FL 32615</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Bradley, Kevin M" sort="Bradley, Kevin M" uniqKey="Bradley K" first="Kevin M." last="Bradley">Kevin M. 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Benner</name><affiliation wicri:level="2"><nlm:aff id="aff1">Firebird Biomolecular Sciences, Alachua, FL 32615, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Firebird Biomolecular Sciences, Alachua, FL 32615</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff2">Foundation for Applied Molecular Evolution (FfAME), Alachua, FL 32615, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Foundation for Applied Molecular Evolution (FfAME), Alachua, FL 32615</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author></analytic><series><title level="j">Analytical Biochemistry</title><idno type="ISSN">0003-2697</idno><idno type="eISSN">1096-0309</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronaviridae (classification)</term><term>Coronaviridae (isolation & purification)</term><term>Coronaviridae (metabolism)</term><term>DNA (metabolism)</term><term>DNA, Single-Stranded (metabolism)</term><term>DNA-Directed RNA Polymerases (metabolism)</term><term>Deoxyribonucleosides (metabolism)</term><term>Fluorescent Dyes (chemistry)</term><term>Hydrogen Bonding</term><term>Immobilized Nucleic Acids (metabolism)</term><term>Limit of Detection</term><term>Microspheres</term><term>Molecular Typing (methods)</term><term>Multiplex Polymerase Chain Reaction (methods)</term><term>Nucleic Acid Heteroduplexes</term><term>Nucleic Acid Hybridization (methods)</term><term>Orthomyxoviridae (classification)</term><term>Orthomyxoviridae (isolation & purification)</term><term>Orthomyxoviridae (metabolism)</term><term>Phycoerythrin (chemistry)</term><term>Pyridones (metabolism)</term><term>RNA, Viral (isolation & purification)</term><term>RNA, Viral (metabolism)</term><term>Respiratory Syncytial Viruses (classification)</term><term>Respiratory Syncytial Viruses (isolation & purification)</term><term>Respiratory Syncytial Viruses (metabolism)</term><term>Respiratory Tract Infections (diagnosis)</term><term>Respiratory Tract Infections (virology)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Synthetic Biology (methods)</term><term>Triazines (metabolism)</term><term>Viral Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN (métabolisme)</term><term>ADN simple brin (métabolisme)</term><term>ARN viral (isolement et purification)</term><term>ARN viral (métabolisme)</term><term>Acides nucléiques immobilisés (métabolisme)</term><term>Biologie synthétique ()</term><term>Colorants fluorescents ()</term><term>Coronaviridae ()</term><term>Coronaviridae (isolement et purification)</term><term>Coronaviridae (métabolisme)</term><term>DNA-directed RNA polymerases (métabolisme)</term><term>Désoxyribonucléosides (métabolisme)</term><term>Hybridation d'acides nucléiques ()</term><term>Hétéroduplexes d'acides nucléiques</term><term>Infections de l'appareil respiratoire (diagnostic)</term><term>Infections de l'appareil respiratoire (virologie)</term><term>Liaison hydrogène</term><term>Limite de détection</term><term>Microsphères</term><term>Orthomyxoviridae ()</term><term>Orthomyxoviridae (isolement et purification)</term><term>Orthomyxoviridae (métabolisme)</term><term>Phycoérythrine ()</term><term>Protéines virales (métabolisme)</term><term>Pyridones (métabolisme)</term><term>RT-PCR</term><term>Réaction de polymérisation en chaine multiplex ()</term><term>Triazines (métabolisme)</term><term>Typage moléculaire ()</term><term>Virus respiratoires syncytiaux ()</term><term>Virus respiratoires syncytiaux (isolement et purification)</term><term>Virus respiratoires syncytiaux (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fluorescent Dyes</term><term>Phycoerythrin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA</term><term>DNA, Single-Stranded</term><term>DNA-Directed RNA Polymerases</term><term>Deoxyribonucleosides</term><term>Immobilized Nucleic Acids</term><term>Pyridones</term><term>RNA, Viral</term><term>Triazines</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Coronaviridae</term><term>Orthomyxoviridae</term><term>Respiratory Syncytial Viruses</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Respiratory Tract Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections de l'appareil respiratoire</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronaviridae</term><term>Orthomyxoviridae</term><term>Respiratory Syncytial Viruses</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN viral</term><term>Coronaviridae</term><term>Orthomyxoviridae</term><term>Virus respiratoires syncytiaux</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Coronaviridae</term><term>Orthomyxoviridae</term><term>Respiratory Syncytial Viruses</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Typing</term><term>Multiplex Polymerase Chain Reaction</term><term>Nucleic Acid Hybridization</term><term>Synthetic Biology</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN</term><term>ADN simple brin</term><term>ARN viral</term><term>Acides nucléiques immobilisés</term><term>Coronaviridae</term><term>DNA-directed RNA polymerases</term><term>Désoxyribonucléosides</term><term>Orthomyxoviridae</term><term>Protéines virales</term><term>Pyridones</term><term>Triazines</term><term>Virus respiratoires syncytiaux</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections de l'appareil respiratoire</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Respiratory Tract Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Hydrogen Bonding</term><term>Limit of Detection</term><term>Microspheres</term><term>Nucleic Acid Heteroduplexes</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biologie synthétique</term><term>Colorants fluorescents</term><term>Coronaviridae</term><term>Hybridation d'acides nucléiques</term><term>Hétéroduplexes d'acides nucléiques</term><term>Liaison hydrogène</term><term>Limite de détection</term><term>Microsphères</term><term>Orthomyxoviridae</term><term>Phycoérythrine</term><term>RT-PCR</term><term>Réaction de polymérisation en chaine multiplex</term><term>Typage moléculaire</term><term>Virus respiratoires syncytiaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Nucleic acid (NA)-targeted tests detect and quantify viral DNA and RNA (collectively xNA) to support epidemiological surveillance and, in individual patients, to guide therapy. They commonly use polymerase chain reaction (PCR) and reverse transcription PCR. Although these all have rapid turnaround, they are expensive to run. Multiplexing would allow their cost to be spread over multiple targets, but often only with lower sensitivity and accuracy, noise, false positives, and false negatives; these arise by interactions between the multiple nucleic acid primers and probes in a multiplexed kit. Here we offer a multiplexed assay for a panel of respiratory viruses that mitigates these problems by combining several nucleic acid analogs from the emerging field of synthetic biology: (i) self-avoiding molecular recognition systems (SAMRSs), which facilitate multiplexing, and (ii) artificially expanded genetic information systems (AEGISs), which enable low-noise PCR. These are supplemented by “transliteration” technology, which converts standard nucleotides in a target to AEGIS nucleotides in a product, improving hybridization. The combination supports a multiplexed Luminex-based respiratory panel that potentially differentiates influenza viruses A and B, respiratory syncytial virus, severe acute respiratory syndrome coronavirus (SARS), and Middle East respiratory syndrome (MERS) coronavirus, detecting as few as 10 MERS virions in a 20-μl sample.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Switzer, C" uniqKey="Switzer C">C. Switzer</name></author><author><name sortKey="Moroney, S E" uniqKey="Moroney S">S.E. Moroney</name></author><author><name sortKey="Benner, S A" uniqKey="Benner S">S.A. Benner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Elbeik, T" uniqKey="Elbeik T">T. Elbeik</name></author><author><name sortKey="Markowitz, N" uniqKey="Markowitz N">N. Markowitz</name></author><author><name sortKey="Nassos, P" uniqKey="Nassos P">P. Nassos</name></author><author><name sortKey="Kumar, U" uniqKey="Kumar U">U. Kumar</name></author><author><name sortKey="Beringer, S" uniqKey="Beringer S">S. Beringer</name></author><author><name sortKey="Haller, B" uniqKey="Haller B">B. Haller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Elbeik, T" uniqKey="Elbeik T">T. Elbeik</name></author><author><name sortKey="Surtihadi, J" uniqKey="Surtihadi J">J. Surtihadi</name></author><author><name sortKey="Destree, M" uniqKey="Destree M">M. Destree</name></author><author><name sortKey="Gorlin, J" uniqKey="Gorlin J">J. Gorlin</name></author><author><name sortKey="Holodniy, M" uniqKey="Holodniy M">M. Holodniy</name></author><author><name sortKey="Jortani, S A" uniqKey="Jortani S">S.A. Jortani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Z" uniqKey="Yang Z">Z. Yang</name></author><author><name sortKey="Chen, F" uniqKey="Chen F">F. Chen</name></author><author><name sortKey="Chamberlin, S G" uniqKey="Chamberlin S">S.G. Chamberlin</name></author><author><name sortKey="Benner, S A" uniqKey="Benner S">S.A. Benner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Johnson, S C" uniqKey="Johnson S">S.C. Johnson</name></author><author><name sortKey="Marshall, D J" uniqKey="Marshall D">D.J. Marshall</name></author><author><name sortKey="Harms, G" uniqKey="Harms G">G. Harms</name></author><author><name sortKey="Miller, C M" uniqKey="Miller C">C.M. Miller</name></author><author><name sortKey="Sherrill, C B" uniqKey="Sherrill C">C.B. Sherrill</name></author><author><name sortKey="Beaty, E L" uniqKey="Beaty E">E.L. Beaty</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nolte, F S" uniqKey="Nolte F">F.S. Nolte</name></author><author><name sortKey="Marshall, D J" uniqKey="Marshall D">D.J. Marshall</name></author><author><name sortKey="Rasberry, C" uniqKey="Rasberry C">C. Rasberry</name></author><author><name sortKey="Schievelbein, S" uniqKey="Schievelbein S">S. Schievelbein</name></author><author><name sortKey="Banks, G G" uniqKey="Banks G">G.G. Banks</name></author><author><name sortKey="Storch, G A" uniqKey="Storch G">G.A. Storch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Benner, S A" uniqKey="Benner S">S.A. Benner</name></author><author><name sortKey="Kim, H J" uniqKey="Kim H">H.J. Kim</name></author><author><name sortKey="Merritt, K B" uniqKey="Merritt K">K.B. Merritt</name></author><author><name sortKey="Yang, Z" uniqKey="Yang Z">Z. Yang</name></author><author><name sortKey="Mclendon, C" uniqKey="Mclendon C">C. McLendon</name></author><author><name sortKey="Hoshika, S" uniqKey="Hoshika S">S. Hoshika</name></author><author><name sortKey="Hutter, D" uniqKey="Hutter D">D. Hutter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Z" uniqKey="Yang Z">Z. Yang</name></author><author><name sortKey="Durante, M" uniqKey="Durante M">M. Durante</name></author><author><name sortKey="Glushakova, L G" uniqKey="Glushakova L">L.G. Glushakova</name></author><author><name sortKey="Sharma, N" uniqKey="Sharma N">N. Sharma</name></author><author><name sortKey="Leal, N A" uniqKey="Leal N">N.A. Leal</name></author><author><name sortKey="Bradley, K M" uniqKey="Bradley K">K.M. Bradley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Glushakova, L G" uniqKey="Glushakova L">L.G. Glushakova</name></author><author><name sortKey="Bradley, A" uniqKey="Bradley A">A. Bradley</name></author><author><name sortKey="Bradley, K M" uniqKey="Bradley K">K.M. Bradley</name></author><author><name sortKey="Alto, B W" uniqKey="Alto B">B.W. Alto</name></author><author><name sortKey="Hoshika, S" uniqKey="Hoshika S">S. Hoshika</name></author><author><name sortKey="Hutter, D" uniqKey="Hutter D">D. Hutter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Geng, H" uniqKey="Geng H">H. Geng</name></author><author><name sortKey="Tan, W" uniqKey="Tan W">W. Tan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dunbar, S A" uniqKey="Dunbar S">S.A. Dunbar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ellis, S E" uniqKey="Ellis S">S.E. Ellis</name></author><author><name sortKey="Coffey, C S" uniqKey="Coffey C">C.S. Coffey</name></author><author><name sortKey="Mitchel, E F" uniqKey="Mitchel E">E.F. Mitchel</name></author><author><name sortKey="Dittus, R S" uniqKey="Dittus R">R.S. Dittus</name></author><author><name sortKey="Griffin, M R" uniqKey="Griffin M">M.R. Griffin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thompson, W W" uniqKey="Thompson W">W.W. Thompson</name></author><author><name sortKey="Shay, D K" uniqKey="Shay D">D.K. Shay</name></author><author><name sortKey="Weintraub, E" uniqKey="Weintraub E">E. Weintraub</name></author><author><name sortKey="Brammer, L" uniqKey="Brammer L">L. Brammer</name></author><author><name sortKey="Cox, N" uniqKey="Cox N">N. Cox</name></author><author><name sortKey="Anderson, L J" uniqKey="Anderson L">L.J. Anderson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jansen, A G" uniqKey="Jansen A">A.G. Jansen</name></author><author><name sortKey="Sanders, E A" uniqKey="Sanders E">E.A. Sanders</name></author><author><name sortKey="Hoes, A W" uniqKey="Hoes A">A.W. Hoes</name></author><author><name sortKey="Van Loon, A M" uniqKey="Van Loon A">A.M. van Loon</name></author><author><name sortKey="Hak, E" uniqKey="Hak E">E. Hak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Karstaedt, A S" uniqKey="Karstaedt A">A.S. Karstaedt</name></author><author><name sortKey="Hopley, M" uniqKey="Hopley M">M. Hopley</name></author><author><name sortKey="Wong, M" uniqKey="Wong M">M. Wong</name></author><author><name sortKey="Crewe Brown, H H" uniqKey="Crewe Brown H">H.H. Crewe-Brown</name></author><author><name sortKey="Tasset Tisseau, A" uniqKey="Tasset Tisseau A">A. Tasset-Tisseau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, T C" uniqKey="Chan T">T.C. Chan</name></author><author><name sortKey="Hsiao, C K" uniqKey="Hsiao C">C.K. Hsiao</name></author><author><name sortKey="Lee, C C" uniqKey="Lee C">C.C. Lee</name></author><author><name sortKey="Chiang, P H" uniqKey="Chiang P">P.H. Chiang</name></author><author><name sortKey="Kao, C L" uniqKey="Kao C">C.L. Kao</name></author><author><name sortKey="Liu, C M" uniqKey="Liu C">C.M. Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bermingham, A" uniqKey="Bermingham A">A. Bermingham</name></author><author><name sortKey="Chand, M A" uniqKey="Chand M">M.A. Chand</name></author><author><name sortKey="Brown, C S" uniqKey="Brown C">C.S. Brown</name></author><author><name sortKey="Aarons, E" uniqKey="Aarons E">E. Aarons</name></author><author><name sortKey="Tong, C" uniqKey="Tong C">C. Tong</name></author><author><name sortKey="Langrish, C" uniqKey="Langrish C">C. Langrish</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tempia, S" uniqKey="Tempia S">S. Tempia</name></author><author><name sortKey="Walaza, S" uniqKey="Walaza S">S. Walaza</name></author><author><name sortKey="Viboud, C" uniqKey="Viboud C">C. Viboud</name></author><author><name sortKey="Cohen, A L" uniqKey="Cohen A">A.L. Cohen</name></author><author><name sortKey="Madhi, S A" uniqKey="Madhi S">S.A. Madhi</name></author><author><name sortKey="Venter, M" uniqKey="Venter M">M. Venter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kehl, S C" uniqKey="Kehl S">S.C. Kehl</name></author><author><name sortKey="Kumar, S" uniqKey="Kumar S">S. Kumar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mahony, J B" uniqKey="Mahony J">J.B. Mahony</name></author><author><name sortKey="Petrich, A" uniqKey="Petrich A">A. Petrich</name></author><author><name sortKey="Smieja, M" uniqKey="Smieja M">M. Smieja</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ginocchio, C C" uniqKey="Ginocchio C">C.C. Ginocchio</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Leland, D S" uniqKey="Leland D">D.S. Leland</name></author><author><name sortKey="Ginocchio, C C" uniqKey="Ginocchio C">C.C. Ginocchio</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hayden, F G" uniqKey="Hayden F">F.G. Hayden</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van De Pol, A C" uniqKey="Van De Pol A">A.C. van de Pol</name></author><author><name sortKey="Van Loon, A M" uniqKey="Van Loon A">A.M. van Loon</name></author><author><name sortKey="Wolfs, T F" uniqKey="Wolfs T">T.F. Wolfs</name></author><author><name sortKey="Jansen, N J" uniqKey="Jansen N">N.J. Jansen</name></author><author><name sortKey="Nijhuis, M" uniqKey="Nijhuis M">M. Nijhuis</name></author><author><name sortKey="Breteler, E K" uniqKey="Breteler E">E.K. Breteler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liao, R S" uniqKey="Liao R">R.S. Liao</name></author><author><name sortKey="Tomalty, L L" uniqKey="Tomalty L">L.L. Tomalty</name></author><author><name sortKey="Majury, A" uniqKey="Majury A">A. Majury</name></author><author><name sortKey="Zoutman, D E" uniqKey="Zoutman D">D.E. Zoutman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y. Chen</name></author><author><name sortKey="Cui, D" uniqKey="Cui D">D. Cui</name></author><author><name sortKey="Zheng, S" uniqKey="Zheng S">S. Zheng</name></author><author><name sortKey="Yang, S" uniqKey="Yang S">S. Yang</name></author><author><name sortKey="Tong, J" uniqKey="Tong J">J. Tong</name></author><author><name sortKey="Yang, D" uniqKey="Yang D">D. Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jansen, R R" uniqKey="Jansen R">R.R. Jansen</name></author><author><name sortKey="Schinkel, J" uniqKey="Schinkel J">J. Schinkel</name></author><author><name sortKey="Koekkoek, S" uniqKey="Koekkoek S">S. Koekkoek</name></author><author><name sortKey="Pajkrt, D" uniqKey="Pajkrt D">D. Pajkrt</name></author><author><name sortKey="Beld, M" uniqKey="Beld M">M. Beld</name></author><author><name sortKey="De Jong, M D" uniqKey="De Jong M">M.D. de Jong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Paris, F" uniqKey="De Paris F">F. de-Paris</name></author><author><name sortKey="Beck, C" uniqKey="Beck C">C. Beck</name></author><author><name sortKey="Machado, A B" uniqKey="Machado A">A.B. Machado</name></author><author><name sortKey="Paiva, R M" uniqKey="Paiva R">R.M. Paiva</name></author><author><name sortKey="Da Silva Menezes, D" uniqKey="Da Silva Menezes D">D. da Silva Menezes</name></author><author><name sortKey="De Souza Nunes, L" uniqKey="De Souza Nunes L">L. de Souza Nunes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Josko, D" uniqKey="Josko D">D. Josko</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Elnifro, E M" uniqKey="Elnifro E">E.M. Elnifro</name></author><author><name sortKey="Ashshi, A M" uniqKey="Ashshi A">A.M. Ashshi</name></author><author><name sortKey="Cooper, R J" uniqKey="Cooper R">R.J. Cooper</name></author><author><name sortKey="Klapper, P E" uniqKey="Klapper P">P.E. Klapper</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Floride</li></region></list><tree><country name="États-Unis"><region name="Floride"><name sortKey="Glushakova, Lyudmyla G" sort="Glushakova, Lyudmyla G" uniqKey="Glushakova L" first="Lyudmyla G." last="Glushakova">Lyudmyla G. Glushakova</name></region><name sortKey="Benner, Steven A" sort="Benner, Steven A" uniqKey="Benner S" first="Steven A." last="Benner">Steven A. Benner</name><name sortKey="Benner, Steven A" sort="Benner, Steven A" uniqKey="Benner S" first="Steven A." last="Benner">Steven A. Benner</name><name sortKey="Bradley, Andrea C" sort="Bradley, Andrea C" uniqKey="Bradley A" first="Andrea C." last="Bradley">Andrea C. Bradley</name><name sortKey="Bradley, Kevin M" sort="Bradley, Kevin M" uniqKey="Bradley K" first="Kevin M." last="Bradley">Kevin M. Bradley</name><name sortKey="Hoshika, Shuichi" sort="Hoshika, Shuichi" uniqKey="Hoshika S" first="Shuichi" last="Hoshika">Shuichi Hoshika</name><name sortKey="Sharma, Nidhi" sort="Sharma, Nidhi" uniqKey="Sharma N" first="Nidhi" last="Sharma">Nidhi Sharma</name><name sortKey="Yang, Zunyi" sort="Yang, Zunyi" uniqKey="Yang Z" first="Zunyi" last="Yang">Zunyi Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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